The DANAGENE DNA Removal Kit provides a method for removal of genomic DNA contamination in RNA preparations using an approach consisting of two sequential filtrations with different MiroSpin columns
DNA contaminating in RNA preparations can serve as a template in PCR to produce a false positive signal from RT-PCR. Although false positives are easily identified by looking at the outcome of a “minus-RT” control.
Specifications:
• Efficient removal genomic DNA from RNA preparations using a 2 column process.
• RNA is isolated without the use of harmful chemicals as phenol or chloroform.
• Rapid protocol 10 minutes.
• Purified RNA can be used in a number of downstream applications incluiding real time PCR, reverse transcription PCR, Notehrn blotting, RNase protection and primer extension, and expression array assays