This kit was designed for total RNA purification from Gram (-) negative bacteria and Gram (+) positive bacteria without using toxic reagents. This RNA Kit includes Bacteria Lysis Buffer and Lysozyme to reduce sample preparation time and minimize hands on time.
The process includes a cell lysis followed by a precipitation of th eproteins and part of genomic DNA. Later, by a precipitation with isopropanol, total RNA is obtained, which is finally totally rehydrated.
Total RNA from different bacteria strains
Total RNA was isolated from different 1 ml cultures of bacterias using the DANAGENE BACTERIA Kit